Chlamydia trachomatis is also responsible for lymphogranuloma venereum. For example, these methods have been useful in the diagnosis of genetic disorders such as sickle cell anemia, &beta -thalassemia and cystic fibrosis. A coamplification PCR assay for the direct detection of both N. gonorrhoeae and C. trachomatis from patients with STD has also been developed. As described here, currently available commercial tests using PCR for the diagnosis of infections include those able to detect C. trachomatis, M. tuberculosis, HIV, herpes simplex virus, cytomegalovirus, enterovirus, hepatitis C virus and other infectious agents. MultiCode-RTx Herpes Simplex Virus 1 & 2 Kit, EraGen Biosciences, Inc. Rapid Identification Test for Mycobacterium tuberculosis complexGen-Probe, Inc.
When methods for microbial genome analysis became available, a new frontier in microbial identification and characterization was opened. Detection of DNA with direct or culture-amplified gene probe technology has been applied to several organisms, including bacteria (14)(15)(16), viruses (17)(18)(19), mycobacteria (20)(21)(22), fungi (23)(24), and even certain parasites (25). Commercial systems for PCR detection of DNA targets of Chlamydia trachomatis and Mycobacterium tuberculosis are manufactured by Roche Molecular Systems (112). 4 Direct probe method of mycobacterial tuberculosis, herpes simplex virus, and Chlamydia trachomatis. Some analyte-specific reagent test kits are available for both herpes simplex virus and enteroviruses, but they still require extensive in-house validation studies. The use of laboratory tests for diagnosis of enteroviral CNS disease was shown to be cost effective long ago 8, but the availability of rapid molecular tests for this purpose has been long coming.
C. trachomatis is also responsible for lymphogranuloma venereum. The PCR technique to detect herpes simplex virus in the cerebrospinal fluid has been used to provide a rapid diagnosis of herpes virus encephalitis. In addition, amplification techniques for Mycobacterium tuberculosis may be used in patients who have a positive smear. A Method to Enumerate Chlamydia trachomatis by Counting DFA Stained Elementary Bodies on a Printed Microscope Slide. A latex agglutination test for the identification of streptococcal groups A, B, C, D, F and G.
Molecular Diagnostics Of Infectious Diseases
Herpes simplex virus (HSV) is a well established cause of nonseasonal viral. The performance of the new Abbott Molecular MTB assay for the qualitative detection of MTB complex using the automated m2000 system or manual sample preparation is summarized in this paper. Tuberculosis (TB) is caused by infection with Mycobacterium tuberculosis (MTB) 1. Detection of Chlamydia trachomatis in symptomatic and asymptomatic populations with urogenital specimens by AMP CT (Gen-probe incorporated) compared to others commercially available amplification assays. ET system for the direct detection of Mycobacterium tuberculosis in respiratory samples. Signal amplification of padlock probes by rolling circle replication. Rapid detection and typing of herpes simplex DNA in clinical specimens by the hybrid capture II signal amplification probe test.