PURPOSE: To Report Two Cases Of Herpes Simplex Virus (HSV) Keratitis After Laser In Situ Keratomileusis (LASIK)

PURPOSE: To report two cases of herpes simplex virus (HSV) keratitis after laser in situ keratomileusis (LASIK). METHODS: Interventional small case series. LASIK in Patients With Herpes Simplex Virus. Herpetic keratitis, if left untreated, can lead to significant corneal scarring and vision loss, and is one of the leading causes of blindness worldwide 1. Two small case studies found no reactivation of HSV after perioperative administration of antiviral therapy in patients with a documented history of ocular HSV infection. Laser in situ keratomileusis in eyes with inactive herpetic keratitis. CASE REPORT A 36-year-old man had bilateral LASIK with a superior hinge in June 2000. 2 ed visual acuity was 20/80 in the right eye and pinhole (PH) 20/40. Infectious keratitis after laser in situ keratomileusis: results of an ASCRS survey.

PURPOSE: To report two cases of herpes simplex virus (HSV) keratitis after laser in situ keratomileusis (LASIK) 2Cases reported Keratitis, Herpetic; Herpes Simplex Keratitis; Herpes Simplex, Ocular. PURPOSE: To report a case of herpes simplex virus reactivation after starting bimatoprost treatment for glaucoma. Two patients who were diagnosed and treated early retained a visual acuity of 0. By Ruth Lapid-Gortzak in Myopia and Cornea. To report two cases of herpes simplex virus (HSV) keratitis after laser in situ keratomileusis (LASIK). Interventional small case series. Reports of keratitis after laser in situ keratomileusis (LASIK), as well as after laser-assisted subepithelial keratectomy (LASEK) or photorefractive keratectomy (PRK), have become increasingly common in recent years, although they are still rare. Post-LASIK herpes simplex virus (HSV)-associated keratitis has been reported with or without previous history of herpetic disease. A peri- and post-operative regimen consisting of systemic aciclovir or valaciclovir 500mg twice daily for one week pre-operatively and two weeks postoperatively and topical aciclovir ointment at bedtime for at least two weeks post operatively in addition to the topical standard post-operative antibiotic treatment is recommended.

Purpose: To report the first multicenter survey in Japan on infectious keratitis after excimer laser surgery. Methods: The laser in situ keratomileusis (LASIK) Safety Network (LSN) Committee sent questionnaires to 28 LSN member hospitals to assess the total number of laser corneal surgeries, the number of infection cases (including suspicious cases), and the postoperative follow-up rate during a 3-year period. Laser in situ keratomileusis (LASIK) is a rapidly evolving ophthalmic surgical procedure. The incidence of thin flaps after LASIK has been reported to vary between 0. Two reports described dislocation of a LASIK flap during vitrectomy surgery. A known history of herpes simplex virus (HSV) is a contraindication for LASIK50 especially in the presence of corneal findings because of the risk of virus reactivation. Culture-proven Herpetic Keratitis After Penetrating Keratoplasty in Patients with No Previous History of Herpes Disease. To report three cases of herpetic infection in recipients of organ-cultured donor corneas among 586 consecutive corneal transplantation procedures. To assess the clinical relevance of tear anti-herpes simplex virus (HSV) antibody measurement for the diagnosis of herpes simplex keratitis.

Cases Reported Keratitis, Herpetic; Herpes Simplex Keratitis; Herpes Simplex, Ocular

Purpose.: To determine corneal sensitivity to selective mechanical, chemical, and thermal (heat and cold) stimulation in patients with a history of herpes simplex virus (HSV) keratitis. Corneal infection by herpes simplex virus (HSV) is a common condition that usually develops as an acute or chronic corneal inflammation. 8 In addition, some patients with active HSV keratitis report sensations of spontaneous pain, discomfort, and dryness. 2. Corneal Collagen Cross-Linking with Riboflavin and UVA. A patient with no history of herpetic keratitis developed herpes simplex keratitis geographical ulcer and iritis five days after treatment 18. In cases of corneal infection after CXL, contact with the infectious agent likely occurred during the early postoperative period rather than during surgery because CXL not only damages keratocytes, but it also kills bacteria and fungi. Delayed post-LASIK microbial keratitis was diagnosed. Immunofluorescence stain was positive for the herpes simplex virus antigen. After excimer laser ablation, the epithelial sheet is repositioned over the treatment zone. Purpose: To report a keratoconic eye that developed severe sterile keratitis and corneal scar after collagen crosslinking necessitating corneal transplantation. Case Report: A 26-year-old man with progressive keratoconus underwent collagen crosslinking and presented with severe keratitis 72 hours after the procedure. One mechanism is infectious etiologies such as herpes simplex virus, 10 Escherichia coli, Staphylococcus epidermidis, and Staphylococcus salivarius. Diffuse lamellar keratitis after corneal crosslinking in a patient with post-laser in situ keratomileusis corneal ectasia. Corneal herpes simplex virus type 1 superinfection in patients with recrudescent herpetic keratitisLies RemeijerRotterdam Eye Hospital, Rotterdam, The NetherlandsInvest Ophthalmol Vis Sci 43:358-63. PURPOSE: To identify localization and kinetics of infiltrated cells and cytokines in murine herpetic keratitis. Experimental laser-assisted in situ keratomileusis induces the reactivation of latent herpes simplex virusD K DhaliwalDepartment of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USAAm J Ophthalmol 131:506-7. To report two cases of herpes simplex virus (HSV) keratitis after laser in situ keratomileusis (LASIK).

Most Cited Taiwan Journal Of Ophthalmology Articles

Laser in situ keratomileusis (LASIK) has become an History of complications in fellow eye increasingly performed procedure throughout the world. PURPOSE: To report two cases of herpes simplex virus (HSV) segments was normal. Recurrences of the herpetic keratitis were seen after tapering of the topical One day postoperatively, UCVA was 6/15 in the steroids; four and three recurrences were observed, respectively. Purpose: To report a case of a dendritic epithelial defect with interface inflammation associated with Alternaria sp. Fungal keratitis is a rare but serious complication of laser in situ keratomileusis (LASIK) surgery. We describe a case of dendritic epithelial defect with interface inflammation associated with Alternaria sp. after LASIK surgery. PURPOSE: To report two cases of herpes simplex virus (HSV) keratitis after laser in situ keratomileusis (LASIK). METHODS: Interventional small case series.

Herpes Simplex Virus-1 (HSV-1) Infection Was Determined By In Situ Hybridization, RT-PCR, And Immunohistochemistry

Herpes simplex virus-1 (HSV-1) infection was determined by in situ hybridization, RT-PCR, and immunohistochemistry 1

Herpes simplex virus type-1 (HSV-1) typically causes infections of the oral mucosa and establishes a lifelong persistence in the sensory ganglia neurons. The presence of chemokines that might attract immune cells and of cytokines that could affect viral replication was determined by quantitative RT-PCR. LAT in Situ Hybridization and Immunohistochemical Findings. Quantitative reverse transcription-PCR (RT-PCR) revealed that LAT+ neurons with or without surrounding T cells were always positive for HSV-1 miRNAs and DNA. However, miRNA expression in every latently infected neuron would provide an additional checkpoint before viral replication is initiated. Herpes simplex virus type 1 (HSV-1) is a human herpesvirus with a specific tropism for sensory neurons. An in situ hybridization for LAT, followed by CD3 immunohistochemistry, was carried out on TG sections as described previously (6, 22, 24). Thus, we have studied herpes simplex virus 1 (HSV-1) infection in the tree shrew trigeminal ganglion (TG) following ocular inoculation. In situ hybridization, PCR, and quantitative reverse transcription-PCR (qRT-PCR) analyses confirm that HSV-1 latently infects neurons of the TG. Immunofluorescence staining with ICP4 monoclonal antibody, and immunohistochemistry detection by HSV-1 polyclonal antibodies, showed a lack of viral proteins in tree shrew TGs during both acute and latent phases of infection.

Herpes simplex virus-1 (HSV-1) infection was determined by in situ hybridization, RT-PCR, and immunohistochemistry 2Herpes simplex virus-1 (HSV-1) infection was determined by in situ hybridization, RT-PCR, and immunohistochemistry. Histopathological analysis showed capillaritis (51 ), plexitis (23 ), nerve hypertrophy (16 ), venulitis (7 ), and fibrosis (3 ). Herpes simplex virus type-1 latency and CD8+ T-cell occurrence were investigated in the trigeminal, geniculate, and vestibular ganglia from seven deceased humans. The difference in CD8 transcript levels among HSV-1 latently infected trigeminal ganglia, geniculate, and vestibular ganglia was less conspicuous. Infection with either HSV-1 or HSV-2 rapidly induced multinuclear giant cell formation and widespread damage in mucosal epithelial cells. The short-term organ culture system provides direct experimental support for the epidemiological findings that pre-existing sexually transmitted infections, including primary and recurrent herpes virus infections at mucosal surfaces, represent major risk factors for acquisition of primary HIV-1 infection. In situ Hybridization and Immunohistochemical Double Labeling. (2004) Real time PCR for monitoring regulation of host gene expression in herpes simplex virus type 1-infected human diploid cells.

To determine whether infectious virus was present after IC inoculation of strain 1716, we performed experiments in immunocompetent Balb/c mice. DNA copies per TG as determined by real-time PCR analysis of purified TG DNA. Chronic brain infection with HSV-1, Chlamydophila pneumoniae, and spirochetes results in complex processes that interact to cause a vicious cycle of uncontrolled neuroinflammation and neurodegeneration. Herpes simplex virus type 1 (HSV-1) and other pathogens including Chlamydophila pneumoniae and Spirochetes are able to infect the brain, evade the host immune response, and are highly prevalent in the AD brain 3 9. Using in situ PCR to detect HSV-1 DNA and immunohistochemistry or thioflavin S staining to detect amyloid plaques, Wozniak and coworkers discovered a striking co-localization of HSV-1 DNA and A within senile plaques in postmortem brains (Fig.

Autoimmune Inflammatory Disease: A Cross-sectional Study

Herpes simplex virus-1 (HSV-1) infection was determined by in situ hybridization, RT-PCR, and immunohistochemistry 3PCR showed the presence of HSV-1 DNA and in situ hybridization showed reactivity with a latency-associated RNA probe, indicating the presence of a non-productive infection. Chronic administration of a high dose of morphine (5 mg day-1) starting 1 month after the ION ligation was shown to reduce mechanical hypersensitivity in CCI-ION. Proenkephalin A mRNA concentrations in the right ganglion of HSVLatEnk-infected rats were comparable to those determined in both trigeminal ganglia of control uninfected animals (not shown). Animals used for RT-PCR, immunohistochemical, or in situ hybridization procedures were killed by decapitation 3 weeks after infection, i. The diagnosis of herpes simplex virus (HSV) infection can be difficult when the characteristic intranuclear inclusions or multinucleated cells, or both, are absent or when the amount of tissue in a biopsy specimen is small. Immunostaining of Kaposi sarcoma latent-associated nuclear antigen 1 is useful to confirm the diagnosis of Kaposi sarcoma, particularly in difficult early lesions or when the neoplasm presents in an unusual location. 25,26 The sensitivity of immunohistochemistry is better than light microscopic identification of viral inclusions, compares favorably with culture and in situ hybridization, and can be completed faster than the shell vial technique with immunofluorescence or culture, allowing for rapid results. Pathogenesis and diagnosis of human meningococcal disease using immunohistochemical and PCR assay. Table 1. Use of Molecular Methods for Diagnosis of Infections of the Central Nervous System. Real-time PCR enables rapid detection of herpes simplex virus-2 DNA in CSF and was found to identify more cases than nest PCR in the group of patients with recurrent meningitis (Franzen-Rohl et al 2007). Detection of the virus in neurons, oligodendrocytes, meningeal cells, ependymal cells, or the blood vessel wall often requires a combination of morphologic, immunohistochemical, in situ hybridization, and PCR methods. CONCLUSIONS PCR and IHC are both sensitive for the detection of HSV-1 in the cornea. (PCR),6-13 immunohistochemistry (IHC),13-15 and in situ hybridisation (ISH),10 16 have been used for the detection of herpes simplex virus type 1 (HSV-1) in the human cornea. This was to determine and compare the sensitivity and specificity of viral culture, the PCR, IHC, and ISH for the detection of HSV-1 in the cornea of patients with and without a history of herpes simplex keratitis (HSK). Herpes simplex virus 1(HSV-1) Central nervous system (CNS) infection Mouse strain dependent effect HSV-1 induced CNS demyelination. In this study, we combine histology, immunohistochemistry, and in-situ hybridization to investigate the relationship between virus and the development of lesions during the early stage ( 24 days PI) of demyelination in different strains of mice. Serial sections were examined by IHC and the number of ‘focal’ areas of viral antigen in the BST and CB per slide determined.

Selective Vulnerability Of Mouse Cns Neurons To Latent Infection With A Neuroattenuated Herpes Simplex Virus-1

Ulcerative dermatitis caused by feline herpes virus 1 (FHV-1) is an uncommon disease characterized by cutaneous ulcers secondary to epidermal, adnexal and dermal necrosis. Sixty-four skin biopsy specimens from cats with compatible lesions were reviewed and tested via PCR and IHC for evidence of FHV-1. Many laboratory methods for the detection of CMV infection have been developed, including serology, viral culture, and molecular methods. Embryo Specimens Stained by Whole Mount In situ Hybridization. Primer and probe sequences used for RT-PCR and in situ hybridization. A recent study applying RT-PCR and immunohistochemical tech-. Measles studies including reverse transcriptase-polymerase chain reaction and viral growth confirmed measles virus, genotype D3. There was no history of recent or remote measles infection or exposure. Polymerase chain reaction (PCR) for herpes simplex virus (HSV)-1 and 2, human herpesvirus-6, enterovirus, Epstein-Barr virus, and arboviruses were negative. Delayed acute measles inclusion body encephalitis in a 9-year-old girl: ultrastructural, immunohistochemical, and in situ hybridization studies.

IHC was finally performed in the samples found positive by RT-PCR, while the 30 SV40-negative thyroid tissues were used as controls.