Mult Scler 2000 Apr;6(2):66-68. Detection of Human HerpesVirus 6 variant A in Multiple Sclerosis Peripheral Blood MonoNuclear Cells Eur Neurol 2000 Apr;43(3):170-173. Other reports have suggested a role for HHV-6 in more severe neurological disease in children, including disseminated demyelination 7 and infarction of the basal ganglia 8. Mononuclear cell inflammation with prominent demyelinative changes was present in all samples except those from normal brains. Detection of active human herpesvirus 6 (HHV-6) infections in CNS tissues from patients with multiple sclerosis (MS) and controls and in patients with MS according to histopathologic appearance. We further addressed the possibility of patient immunosuppression contributing to their HHV-6 positivity by comparing the incidence of active HHV-6 infections in peripheral blood lymphocytes of patients with MS with that in peripheral blood lymphocytes from bone marrow and liver transplant recipients. These findings suggest that HHV-6 variants might be responsible for specific infection patterns in glial cells in vivo. 2000. Detection of human herpesvirus 6 variant A in peripheral blood mononuclear cells from multiple sclerosis patients. Eur.
We here report the antigenic epitope of HHV-6 p41 recognized by this MAb. All HHV-6 isolates can be classified into either of the two major viral variants, designated HHV-6A and HHV-6B, with distinctive genetic, immunologic, and biological traits (2). (22) reported that HHV-6 DNA sequences were found by PCR in peripheral blood mononuclear cells from 7 of 34 MS patients and 2 of 6 patients with idopathic transverse myelitis, but none of 20 healthy controls. (2000) Detection of human herpesvirus 6 variant A in peripheral blood mononuclear cells from multiple sclerosis patients. Human herpesvirus 6 (HHV-6) is the common collective name for Human herpesvirus 6A (HHV-6A) and Human herpesvirus 6B (HHV-6B). A variety of tests are used in the detection HHV-6, some of which do not differentiate the two species. Gallo cultivated peripheral blood mononuclear cells from patients with AIDS and lymphoproliferative illnesses. (1992) described two very similar, yet unique variants: HHV-6A and HHV-6B. Official Full-Text Publication: Environmental triggers of multiple sclerosis on ResearchGate, the professional network for scientists. Institute of Experimental Immunology, Department of Neuroinflammation, University of Z rich, Z rich, Switzerland.
Human herpesvirus 7 (HHV-7) was first isolated in 1990 from the CD4+ T cells of a healthy individual whose activated cells in culture showed cytopathic effects. J Virol 2000; 74:4530. HHV-7 and HHV-6 variants A and B. New Microbiol 1997; 20:187. Modulatory effects of human herpes virus-7 on cytokine synthesis and cell proliferation in human peripheral blood mononuclear cell cultures. IgG subclasses and DNA detection of HHV-6 and HHV-7 in healthy individuals. Multiple sclerosis (MS) is a T cell-mediated autoimmune disease that is triggered by unknown exogenous agents in subjects with a specific genetic background. A recent neuropathological analysis of MS lesions has shown a demyelination pattern that appears to be induced primarily by a functional disturbance of oligodendrocytes. An association between HHV-6, a beta herpesvirus with a seroprevalence of 72 to 100 percent in healthy adults worldwide, and MS has been suggested by the demonstration of viral antigen in oligodendrocytes of MS white matter lesion but not in control brain (23). There are two variants of HHV-6, variant A and variant B (HHV-6A and HHV-6B, respectively). The virus then replicates, assembles, and exits the infected cell to infect other cells. HHV-6 DNA sequences have been detected in peripheral blood mononuclear cells of as many as 90 of one study population 23. Eur Neurol 2002; 48: 234-235 PubMed DOI.
Definition Of A Divergent Epitope That Allows Differential Detection Of Early Protein P41 From Human Herpesvirus 6 Variants A And B
Human herpesvirus 6 (HHV-6) was the sixth herpesvirus discovered. Isolated in 1986 during attempts to find novel viruses in patients with lymphoproliferative diseases, HHV-6 is now recognized as a T-cell lymphotropic virus with high affinity for CD4 lymphocytes. A beta herpesvirus (like cytomegalovirus CMV and human herpesvirus 7 HHV-7 ), HHV-6 comprises 2 forms, A and B; as of 2012, HHV-6A and HHV-6B are officially considered distinct species rather than variants of 1 species. Multiple sclerosis (MS). DNA load in plasma, peripheral blood mononuclear cells (PBMCs), and tissues was evaluated by using a calibrated quantitative real-time polymerase chain reaction (PCR) assay. HHV-6 variant A encodes a distant chemokine homolog, U83A, and a polymorphism promoting a secreted form was identified. Detection of human herpesvirus 6 variant A in peripheral blood mononuclear cells from multiple sclerosis patients. Eur. Neurol. April 15, 2016, 196 (8). IFNs are cytokines produced by mononuclear cells in response to viral infection. With this information in mind, in the present work we aimed at: first, replicating the effect of the two top-associated SNPs originally described12 in three independent cohorts from Spain, in order to quantify the effect size of these polymorphisms in MS predisposition; second, characterizing whether the response of MS patients to IFN- therapy is conditioned by those IRF5 polymorphisms and third, evaluating whether there is an association between HHV-6 infection and the IRF5 variants. The etiology of the neurogenerative disease multiple sclerosis (MS) is unknown. Herpesviruses can activate HERVs, and HERVs are activated in MS patients. Both envelope proteins were detected on B cells and monocytes only, with the expression of HERV-H Env epitopes generally higher than the expression of HERV-W Env epitopes. 2000, 101: 229-238. Miller Fisher Syndrome (MFS) is a rare variant of Gulliain Barre syndrome (GBS) characterized by external ophthalmoplegia, ataxia, areflexia, and usually by positive anti GQ1b antibody. There are a few publications reporting association of GBS with HHV-6. HHV-6 DNA with PCR was detected in the cerebrospinal fluid (CSF) of a 59 year-old female patient diagnosed with MFS/pharyngeal-cervical-brachial variant of GBS overlap from clinical findings and positive anti-GQ1b antibody in the serum. HHV-6A and HHV-6B may infect several neural cells and some studies have shown concurrence with MS and progressive multifocal leukoencephalopathy(44). Eur Neurol. Sclerosis Peripheral Blood MonoNuclear Cells Eur Neurol 2000 Apr; 43 (3): 170-173.
Human Herpesvirus 7 Infection
Immunoglobulin class-switched B cells form an active immune axis between CNS and periphery in multiple sclerosis. Sci Transl Med. 2014 Aug 6; 6(248):248ra106. Neuropathology in multiple sclerosis: new concepts. In this review evidence is discussed for a pathogenetic role of demyelinating antibodies, toxic macrophage products, cytotoxic T-cells as well as metabolic disturbances of oligodendrocytes. The more distressed our respondents were during the war, the more distressed our respondents were during the war, the more likely they were to employ a variety of ways of coping. 2000 Apr 24. DNA of peripheral blood mononuclear leukocytes (PBL) was isolated and amplified by polymerase chain reaction techniques. HHV-6 DNA was detected in 7 of MS patients and in 14 of controls. Frequent HHV-6 reactivation in Multiple Sclerosis and Chronic Fatigue Syndrome patients. Chlamydia, human herpes virus-6) in blood of chronic fatigue syndrome patients: association with signs and symptoms. Seventy percent of the HHV-6 isolates from CFS patients were Variant A, similar to those reported in AIDS It has already been shown that active HHV-6 infection in HIV-infected patients enhanced the AIDS disease process. Lusso, Paolo et al.; Infection of Natural Killer Cells by Human Herpesvirus 6; Frequent HHV-6 reactivation in Multiple Sclerosis and Chronic Fatigue Syndrome patients. Detection of Human Herpesvirus 6 in Plasma of Children with Primary Infection and Immunosuppressed Patients by Polymerase Chain Reaction. His team had looked at HHV6 in plasma, CSF and white blood cells. Seventy percent of the HHV-6 isolates from CFS patients were Variant A, similar to those reported in AIDS It has already been shown that active HHV-6 infection in HIV-infected patients enhanced the AIDS disease process. 2000 Sep;8(9):410-8.