Diagnosis of Herpes Simplex Virus in the Era of Polymerase Chain Reaction on ResearchGate, the professional network for scientists. Polymerase chain reaction in neonatal HSV encephalitis: an assay to count on? Diagnosis of herpes simplex virus in the era of polymerase chain reaction. Beyond the neonatal period, most primary HSV-1 infections occur in infancy and childhood and are transmitted primarily by contact with infected saliva. Polymerase chain reaction (PCR) is the preferred diagnostic method for herpes simplex virus CNS infection 11, 12, 13 and is likely valuable (on serum) in disseminated disease, as well. Overall sensitivities of PCR in neonatal herpes simplex virus disease range from 75-100, with overall specificities of 71-100.
Natural history of neonatal herpes simplex virus infections in the acyclovir era. PCR testing for the diagnosis of herpes simplex virus in patients with encephalitis or meningitis. Rapid diagnosis of herpes simplex encephalitis by nested polymerase chain reaction assay of cerebrospinal fluid. In the case of herpes simplex virus (HSV) encephalitis in adults, the virus is almost never detectable in cerebrospinal fluid (CSF) by culture. As a result of these problems, prior to the widespread use of CSF PCR testing in viral diagnosis, patients were often treated with unnecessary empiric antimicrobial therapy for prolonged periods and underwent invasive diagnostic procedures, such as brain biopsy.
Polymerase chain reaction for diagnosis of genital herpes in a genitourinary medicine clinic. Results: 109 patients (46) had a positive test for herpes simplex virus (HSV). Over the study period, 236 patients agreed to participate and paired specimens were available from all for analysis. HSV DNA was amplified in the CSF of this patient by PCR; serologic tests (seroconversion by complement fixation) and electroimaging tests (electroencephalography and computerized tomography) supported the diagnosis (80). Episodes last a few days and may recur over a period of months or years (98, 108). (1993) Use of polymerase chain reaction for laboratory diagnosis of herpes simplex virus encephalitis. Real-Time Polymerase Chain Reaction Detection of Herpes Simplex Virus in Cerebrospinal Fluid and Cost Savings from Earlier Hospital Discharge. Patients discharged between 2 to 10 days after admission were included in the study, because it was possible that test results could have influenced a discharge decision within this time period.
Challenges In The Diagnosis And Management Of Neonatal Herpes Simplex Virus Encephalitis
Genital herpes infection is common in the United States. The average incubation period after exposure is 4 days (range, 2 to 12). Rates have risen over the period of a decade. DNA detection using polymerase chain reaction (PCR) of a swab from the base of an ulcer. Herpes simplex virus 2 (HSV-2) is the main cause of genital herpes. Most new cases of genital herpes infection do not cause symptoms, and many people infected with HSV-2 are unaware that they have genital herpes. During inactive periods, the virus cannot be transmitted to another person. Polymerase chain reaction (PCR) tests are much more accurate than viral cultures, and the CDC recommends this test for detecting herpes in spinal fluid when diagnosing herpes encephalitis (see below). Utilizing polymerase chain reaction (PCR) technology, HSV DNA can be detected from genital swab specimens from HSV-2 seropositive women on 28 of days (239). At most, only 2-6 of patients recovering from neonatal SEM disease will experience any neurologic sequelae if they receive optimal diagnostic and therapeutic support during the acute period.